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Leaf Pattern Design

Protein Gel Electrophoresis

Protein Gel Electrophoresis

(can also employ variations for detecting LPS, etc.)

Convenient printable template here from our printable "Templates" page for convenient logging of samples in 15-lane gels (click here).

Possibly useful example for approx [protein] by gel.

Typically use Abs280, but occasionally this is useful.

BSA_Quantity_Standards-small.jpg

Rough estimation of protein quantification using BSA.

Time for a protein gel refresher or intro before you do this the first time? Luckily for biochem type exits: videos and tutorials abound (on Google, You Tube, JoVE)!

SDS-PAGE-Google-pic.jpg
JoVE video here - part of "Basic Methods" videos. (Stanford maintains JoVE subscription.)
JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Separating Protein with SDS-PAGE. JoVE, Cambridge, MA, (2023).
JoVE-runningGel.jpg
Companies that sell gels also have their own tutorial videos and written packets. Google: "Invitrogen protein gel instructions" or "different protein gels" or related phrases to find what you are interested in. Other vendors with files and pages on this are BioRad and Thermo Fisher.
If you have questions or would like to have help, ask a lab member!  No lab member to help on this? Ask Lynette. Lynette has run hundreds of protein gels (and enjoys it!). Same for Westerns.
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